TB-500

Description

TB-500 is not thymosin beta-4 in miniature; it is the piece of thymosin beta-4 that does the work. The parent protein runs 43 amino acids. The synthetic compound researchers actually use is a 17-residue fragment built around the LKKTETQ motif. If you have encountered thymosin beta-4 in the actin-dynamics literature, you have already encountered the active site of TB-500.

Proposed Mechanism

The mechanism is straightforward to describe and hard to stop thinking about: G-actin sequestration. By binding monomeric actin and regulating the polymerization rate, the peptide shifts cellular migration kinetics in assays across tissue types. Sosne et al. (2007) characterized the actin-binding domain at positions 17-23 of the parent protein, which is the exact motif the synthetic fragment reproduces. In cardiac work, Bock-Marquette et al. (2004) reported findings that drew the peptide into regenerative medicine research; later corneal and dermal work extended the mechanism into epithelial contexts.

Research Literature

The 2004 Nature paper from Bock-Marquette’s group is the citation most researchers reach for first. Sosne et al. (2007) is the structural follow-up. Crockford et al. (2010) reviewed the preclinical evidence base, noting how consistently the cellular-migration phenotype reproduced across tissue types. Goldstein and Hannappel (2011, Annals NYAS) extended the review context across cardiac, neural, and dermal models. Human pharmacokinetic data remains limited to early-phase work. The LKKTETQ motif carries the binding activity of the full 43-residue protein, which is what makes the fragment a practical tool compound.

Further reading: PubMed references for this compound.

Research Applications

Used in dermal tissue-repair research, corneal epithelial migration assays, cardiac tissue remodeling models, and actin-cytoskeleton dynamics studies. Frequently co-administered with BPC-157 in multi-pathway tissue-research protocols.

Supplied strictly for laboratory research use. Not for human or veterinary use.

Reconstitution

Supplied as a lyophilized powder. Reconstitute with bacteriostatic water prior to use. Use the Peptigo reconstitution calculator for guidance on volume and concentration.

Storage

• Unopened: room temperature up to 30 days during transit; refrigerate at 2-8°C long-term.
• Reconstituted: refrigerate at 2-8°C; stable 4-6 weeks. Protect from light.

Quality Verification

Every batch is independently tested by Janoshik Analytical. Certificates of Analysis include HPLC-UV purity, LC-MS identity confirmation, LAL bacterial endotoxin quantification, and USP <71> sterility testing.

Not a drug, supplement, or cosmetic. For in vitro research use only. Not for human consumption.

Frequently Asked Questions

Is this product legal to purchase in Canada for research use?

Peptigo supplies all products strictly for laboratory and in vitro research use. The compound is not a drug, supplement, or cosmetic, and is not sold for human or veterinary use. Researchers purchasing for their own laboratory work, university research programs, or independent in vitro protocols are the intended customer base. Health Canada’s April 2026 advisory specifically addresses unauthorized injectable peptide use; the research-use framing of this product is independent of that consumer-use category.

How is purity verified, and where can I see the Certificate of Analysis?

Every batch is tested independently by Janoshik Analytical, an accredited third-party laboratory. The standard testing panel includes HPLC-UV purity verification, LC-MS identity confirmation, LAL bacterial endotoxin quantification, and USP sterility testing. The Certificate of Analysis for each batch is available on the product page after purchase, and the testing methodology is detailed on the Lab Testing reference page.

What is the standard reconstitution and storage protocol for laboratory use?

The compound is supplied as a lyophilized powder. The standard diluent for reconstitution in research protocols is bacteriostatic water (0.9% benzyl alcohol). The Peptigo reconstitution calculator handles the volume-and-concentration arithmetic for a given target research dose. Unopened lyophilized peptide is stable at room temperature for up to 30 days during transit and should be refrigerated at 2-8°C for long-term storage. Reconstituted peptide should be refrigerated at 2-8°C and is typically functional for 4-6 weeks; protect from light.

Frequently Asked Questions

What is TB-500?

TB-500 is a synthetic peptide fragment related to thymosin beta-4. In animal-model research it is studied for its effects on cell migration and tissue-repair processes, which is why it is often paired with BPC-157 in what researchers nickname the “Wolverine” combination.

TB-500 vs BPC-157, how do they differ?

They are studied for different mechanisms: BPC-157 research focuses on angiogenesis (new blood-vessel formation), while TB-500 research focuses on cell migration and actin dynamics. We compare them directly in BPC-157 vs TB-500.

How is TB-500 reconstituted in research?

Lyophilized TB-500 is reconstituted with bacteriostatic water before use in the lab. Our reconstitution guide walks through the standard protocol, and the dosage calculator handles the volume math.

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