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Reconstitution cheat sheet.

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Research Peptides · Canada

Peptide Reconstitution Cheat Sheet

For laboratory research use only. Math examples — not dosing advice.

Quick math

Concentration (mg/mL) = vial mass (mg) ÷ recon volume (mL)

Volume per dose (mL) = desired dose (mg) ÷ concentration (mg/mL)

Insulin syringe units (U-100) = volume per dose × 100

Worked example

BPC-157 5 mg vial + 2 mL bacteriostatic water → 2.5 mg/mL.
For a 0.25 mg dose: 0.25 ÷ 2.5 = 0.1 mL → 10 units on a U-100 syringe.

Storage

  • Refrigerate at 2–8°C after reconstitution
  • 28-day window with bacteriostatic water
  • Don’t freeze (ice crystals damage peptides)
  • Protect from light
  • Discard after the 28-day window

Reconstitution rules

  • Wipe both vial septums with 70% alcohol — air-dry
  • Inject water down the inner wall of the vial — never directly at the lyophilized cake
  • Swirl gently for 30–60 seconds — never shake
  • Use a fresh alcohol pad before each subsequent draw

Quick reference (U-100 units for 0.25 mg)

PeptideVialReconConc.U-100
BPC-1575 mg2 mL2.5 mg/mL10 u
TB-5005 mg2 mL2.5 mg/mL10 u
Wolverine (BPC/TB)10 mg2 mL5.0 mg/mL5 u
KLOW (4-blend)80 mg5 mL16 mg/mL2 u
KPV10 mg2 mL5.0 mg/mL5 u
Thymosin A-15 mg2 mL2.5 mg/mL10 u
Selank10 mg2 mL5.0 mg/mL5 u
Semax10 mg2 mL5.0 mg/mL5 u
CJC-1295 (No DAC)10 mg2 mL5.0 mg/mL5 u
CJC-1295 + DAC2 mg2 mL1.0 mg/mL25 u
Ipamorelin5 mg2 mL2.5 mg/mL10 u
Tesamorelin10 mg2 mL5.0 mg/mL5 u
IGF-1 LR31 mg1 mL1.0 mg/mL25 u
Semaglutide5 mg2 mL2.5 mg/mL10 u
Tirzepatide10 mg2 mL5.0 mg/mL5 u
Retatrutide10 mg2 mL5.0 mg/mL5 u
Cagrilintide5 mg2 mL2.5 mg/mL10 u
NAD+500 mg5 mL100 mg/mL<1 u
GHK-Cu50 mg5 mL10 mg/mL3 u
Glow (3-blend)70 mg5 mL14 mg/mL2 u
Melanotan II10 mg2 mL5.0 mg/mL5 u
Live calculator + how-to guide: peptigopeptides.com/calculator/
For laboratory research use only · Not for human or veterinary administration